Osteoarthritis (OA) is the most common chronic joint disease and it may progressively cause disability and compromise quality of life. Lately, the role of single cell RNA sequencing in the pathogenesis of OA has drawn a lot of attention. Number of literatures were studied, reviewed and analysed to write this review. In this review, use and knowledge on sc-RNA in OA will be outlined. Overall, the use of scRNAseq has enabled a more comprehensive understanding of the diverse cell populations and molecular signaling pathways involved in osteoarthritis pathogenesis.